Gel entrapment of enzymes

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Polyacrylamide Gel Entrapment of Adenylate Kinase and Acetate Kinase

The factors that limit the stability of adenylate kinase and acetate kinase in solution have been examined and compared with those that determine stability under conditions encountered during photochemically initiated polymer gel formation in solutions of acrylamide and N,N'-methylene-bisacrylamide. Both adenylate kinase (from rabbit and pig muscle) and acetate kinase (from E. Coli) contain cys...

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Entrapment of enzymes and nanoparticles using biomimetically synthesized silica.

Entrapment of enzymes and nanoparticles using biosilicification reactions.

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Computer-controlled automation of radioimmunoassay based on gel entrapment of antibody.

We describe a new, broadly applicable approach to radioimmunoassay. Solid-phase binding reagent is prepared by entrapping antibody in polyacrylamide gel. This gel is then fragmented, sieved, and dehydrated in 95 percent ethanol. Upon evaporation of the ethanol, the dry antibody-gel particles are dispensed into miniature disposable plastic columns. A unidirectional flowing stream configuration i...

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Gel entrapment of antibody: a new strategy for facilitating both manual and automated radioimmunoassay.

A technique of radioimmunoassay is presented that eliminates pipetting and centrifugation, and excludes interferences by high-molecular-weight materials from the incubation and separation steps. A solidphase binding reagent is prepared by first entrapping antibody in polyacrylamide gel. This gel is then fragmented, sieved, dried with ethanol or lyophilized, and placed in miniature disposable ch...

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The sol-gel entrapment of noble metals in hybrid silicas: a molecular insight

BACKGROUND Why are metal nanoparticles sol-gel entrapped in ORMOSIL so active and stable? In other words, why ORMOSIL-entrapped metal nanoparticles are more active and selective than many heterogenized counterparts, including silica-entrapped noble metals? RESULTS Unveiling specific interactions between MNPs and the molecular structure of ORMOSIL, this work investigates subtle structural aspe...

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ژورنال

عنوان ژورنال: Biotechnology and Bioengineering

سال: 1972

ISSN: 0006-3592,1097-0290

DOI: 10.1002/bit.260140514